Effects of Peripartal Sertraline on Maternal Calcium Metabolism and Maternal Bone Health

Abstract Lactation taxes mothers’ bone health, causing them to lose up 10% of their mass for milk calcium. Selective serotonin reuptake inhibitor antidepressants (SSRIs) are commonly used during pregnancy and lactation but independently decrease mass. We previously demonstrated that both maternal offspring densities were compromised by treatment with the SSRI fluoxetine throughout lactation. In this study, we examined whether sertraline similarly affect bone. Female C57BL/6 dams (n=23/group) treated (10 mg/kg/d) or a vehicle (DMSO) 41 d (E0-lactation(L) 21). Pups euthanized at weaning (L21). Dams peak (L10, n=4/group), (L21, n=9/group) 3 mo post-weaning (n=10/group) analysis. Sertraline did not dams’ weight gain impact production/consumption, measured weigh-suckle-weigh. Unlike fluoxetine, which increases circulating calcium, reduced serum calcium concentration L21 compared control (p<0.05). Mammary genes altered L10, observed decreased mammary expression transporters Orai1, Spca1, Spca2, Serca2 (p<0.05), tendency Pthlh (a calcium-regulating peptide; p=0.08). intestinal duodenum, Calb1 cytosolic trafficking protein) was increased L10 Trpv6, Pmca1 Spca2 in either tissue, although gland 3.5 times duodenum. Previously, reported markers formation (P1NP) resorption (CTX) unchanged sertraline-treated L21. This is unlike P1NP without altering CTX. Furthermore, control, alter (e.g. Trap, Rank/RankL, Alp, Runx2) femoral post-weaning. Interestingly, utero exposure litter size (5.4 vs 6.8 pups/dam, p<0.01) pup mortality (20% 5% dead pups/litter, first 24 hours postpartum controls, length gestation live birth rate. Exposure surviving offspring, nor it pups weaning, suggesting an adequate supply from dam. These data suggest that, high-dose low-dose peripartal has milder on may reduce could induce failure thrive offspring.